Analysis of fungal communities via metabarcoding (ITS rDNA sequences) data, examining how communities differ between benthic substrates and across a stream continuum and annual flow permanence gradient.

Analysis of fungal community metabarcoding (ITS rDNA) data, examining how communities differ between benthic substrates and across a stream continuum and annual flow permanence gradient.

ungal ITS1 rDNA amplicon metabarcoding analysis, examining effects of water presence, annual flow permanence, and connectivity on fungal community composition and extracellular enzyme activity in decaying leaf litter, epilithic biofilms, and sediments from 50 sites covering a natural streamflow permanence gradient in a tallgrass prairie stream network.

Bioinformatics pipeline (Rmd file knitted to html) with R code + some shell for the Konza Synoptic ITS1 fungal community metabarcoding. Starts with fastq files, end with taxonomy and count tables + fasta for fungal ASVs.